To further assess the effects of IER5-mediated Cdc25B reduction on colony formation of ALDHhi/CD34+ cells from an AML patient (M1), we investigated whether transfection of Cdc25B DNA could rescue the colony forming activity of the AML-derived ALDHhi/CD34+ cells transfected with IER5 DNA or treated with TMPP. The gene discussed is IER5; the disease is acute myeloid leukemia.