In addition, to confirm that IER5-mediated-inhibition of colony formation of AML-derived ALDHhi/CD34+ cells from patients was mediated by an effect on transcription of the IER5 target gene, we assayed the mRNA expression of the IER5 target gene Cdc25B in AML-derived ALDHhi/CD34+ cells. The gene discussed is IER5; the disease is acute myeloid leukemia.