To determine the transcriptional regulatory mechanisms that associate with elevated Kpnβ1 and Kpnα2 expression in cancer and transformed cells, an approximate 2 Kb region upstream of the transcription start site of the Kpnβ1 and Kpnα2 genes, together with approximately 100 bp of their 5′ untranslated regions (downstream from the transcription start site), were separately cloned into pGL3-Basic for promoter analysis. This evidence concerns the gene KPNB1 and cancer.