In order to investigate the role of individual K8 phosphorylation (Ser73 and Ser431) during tumor progression in OSCC, shRNA-resistant K8-Ser73Ala and K8-Ser431Ala phospho-mutants were transfected in K8 deficient AW13516 C1-shRNAK8.2 cells. The gene discussed is KRT8; the disease is neoplasm.