To examine in more detail the effect of amphiphysin-2 knockdown HeLa cells were infected with a luciferase marker-expressing virus SFV-RLuc using two different m.o.i (0.5 and 5), and the replication of this virus was monitored at several time-points after infection based on the luciferase activity in the infected cultures pretreated with amphiphysin-2 or control siRNAs. The gene discussed is BIN1; the disease is infection.