It has been shown previously that the ALT pathway does not substitute for telomerase in the process of tumorigenesis in vivo[23]; therefore, we established the tumor xenograft by subcutaneous injection of 1×106 T24, T24/PCI, or surviving T24/DN868A cells into 6–8-week-old nude mice (n = 4 mice per group). This evidence concerns the gene GPT and neoplasm.