TP53 and cancer: Numerous other temperature-sensitive mutations were later identified and targeted for restoration.[17] Ishioka's group alone assessed a collection of over 2,000 p53 mutants for temperature sensitivity and identified 113 mutants with activity at 303 K.[18] This represents about ∼10% of all reported single amino acid alterations of the DBD in human cancers.[3] Here, we focused on the R248Q mutant, which is the most frequently occurring mutation in human cancer.