There was significantly elevated VDR nuclear staining as compared with melanomas with <10% of cells with nuclear NF-κB. The relationship of cytoplasmic VDR immunostaining and translocation of NF-κB into nucleus was less clear, because the lowest cytoplasmic VDR immunostaining was seen in melanomas with <10% and 50.1–75% of cells with nuclear NF-κB (Figure 7D). This evidence concerns the gene NFKB1 and melanoma.