For all node-negative HNSCC patients low YB-1 protein expression is associated with higher 5-year DSS rate, which is statistically significant for nuclear YB-1 protein expression at the IF (N0, P=0.001) and supported for the nuclear plus cytoplasmic co-expression of YB-1 demonstrating a trend without statistical significance (Table 4). This evidence concerns the gene YBX1 and head and neck squamous cell carcinoma.