RCL1 and fascioliasis: The HF135 (Millipore) NC membranes, which allow flow rates of 135±34 sec/4 cms, a pad conjugate containing rpCL1 bound to colloidal gold at pH 8.6, together with protein A in the test line, and mAb MM3 in the control line, were used to construct a lateral diffusion test for serodiagnosis of human fasciolosis.