Methylation-specific PCR (MSP) results showed that PRDM5 was frequently methylated in silenced cell lines, including 80% (4/5) nasopharyngeal, 44% (8/18) esophageal, 76% (13/17) gastric, 25% (3/12) hepatocellular, and 50% (2/4) cervical carcinoma cell lines (Figure 2, Table 1), with the exception of only infrequent PRDM5 methylation detected in lung, colon, ovarian and bladder cancer cell lines (Figure S1). Here, PRDM5 is linked to urinary bladder cancer.