However, we neither detected any significant difference in GPC3 mRNA expression between the HCC and control groups, nor have we found any relationship between the GPC3 expression in PB cells and the tumor recurrence after OLT which suggested that GPC3 mRNA test in PB does not have the same diagnostic value as that of measurement of GPC3 protein level in plasma[16][17][36]. This evidence concerns the gene GPC3 and hepatocellular carcinoma.