To clarify whether euphol could also diminish the production of inflammatory mediators or modify the profile of cytokines produced by the inflammatory cells present in colonic tissue after inflammatory stimulus, we cultured primary bone marrow-derived macrophages and evaluated the influence of euphol on MCP-1, TNF-α, IL-6, IFN-γ and IL-10 production after in vitro stimulation with LPS (1 μg/ml, for 24 h), an important component of colitis-induced damage. The gene discussed is CCL2; the disease is colitis.