APC and hepatitis A virus infection: In this study, we performed comprehensive bisulfite-specific PCR (BSP) sequencing of the sense and antisense strands of 250 base pairs (bp) of the promoter and the first exon region of the APC gene followed by quantitative methylation-specific PCR assays of both DNA strands on DNA isolated from HCC, matched adjacent non-HCC, cirrhosis, hepatitis, and normal liver tissues.