To confirm that existence of antisense strand-biased methylation of the APC gene in normal liver resulted in inconsistent HCC specificity, as suggested by BSP sequencing, and to evaluate the specificity of mAPC sense and mAPC antisense as potential biomarkers of HCC, we developed quantitative MSP assays for the sense strand (APC_MSP_S) and antisense strand (APC_MSP_AS) of the APC gene. Here, APC is linked to hepatocellular carcinoma.