No significant difference was found between low (grade 1) and high (grade 2 and 3) tumor grades (P = 0.401), AFP-negative and AFP-positive tumors (P = 0.963), HBV-infected and non-HBV-infected HCC (P = 0.985), and/or HCV status (P = 0.445). The gene discussed is AFP; the disease is hepatocellular carcinoma.