Figure 5b shows that while the 26 KDa band, representing deglycosylated full length PrP was detected by RTC in all samples, the TgMHu2ME199K samples presented additional and unique deglycosylated bands (see arrows), different also in their molecular weight from the 19 Kda band representing deglycosylated PK resistant PrP in scrapie brains (lane 5). This evidence concerns the gene PRNP and scrapie.