To investigate the inverse correlation of E-cadherin with CD44 in esophageal cancer cell lines, we analyzed esophageal squamous cell carcinoma cell lines (TE-1, -7, -8, -11 and -12), head-and-neck squamous cancer cell lines (JHU-012, JHU-013), as well as adenocarcinoma cell lines (FLO-1, OE33 and SK-GT-4) by Western Blot for E-cadherin and CD44 expression as well as TGFβRII and vimentin. This evidence concerns the gene CD44 and esophageal cancer.