CASP3 and Alzheimer disease: In order to rule out the possibility that isoflurane itself might not be activating caspase-3 above basal levels in WT and AD Tg mice, we assessed the effects of isoflurane alone on caspase-3 activation in the mice and we were able to show that anesthesia with 1.4% isoflurane for six hours induced caspase-3 activation in both WT and AD Tg mice (Figure S2A, S2B, S2C and S2D).