Therefore, in order to investigate this possibility, sensitive and resistant CRC MIP101 cells were transiently transfected with vectors containing only the N-terminus (SP-N), the follistatin-like (SP-F), or the C-terminus (SP-C) domains of SPARC, or mutant domains (SP-Nmut1, SP-Nmut2, SP-Fmut1, SP-Fmut2) (Fig. 1A). The gene discussed is SPARC; the disease is colorectal carcinoma.