APC and neoplasm: APC was previously observed with 10% of methylation in tumours and 0% in NT, but both positive tumours were NSE (Koul et al, 2002), and no APC methylation has been reported in Ses; it is important to emphasise that this study was performed using conventional MSP, a less sensitive technique than QMSP used in the present study (Hoque et al, 2004), which may explain the different percentages observed.