In addition, we also observed that MUC16 expressing cell lines, such as Capan 1 (liver met), Colo 357 (lymph node met) and T3M4 (lymph node met) were derived from metastatic sites while the MUC16 non expressing cell lines such as Panc1, AsPC1 and BxPC3 were isolated from the primary tumor site (pancreas). The gene discussed is MUC16; the disease is neoplasm.