HF dose-dependent up-regulation of TGFβ (P<0.001), TGFβRI (P<0.001), SMAD3 (P<0.001), p15 (P<0.001), and p21 (P<0.001) gene expression was detected after 72 hours of incubation. The gene discussed is TGFB1; the disease is hydrops fetalis.