Annexin V-FITC/propidium iodide staining followed by flow cytometric analysis of apoptosis in C6, U251 and U87 glioma cells treated with LY294002 (10 μmol/L), or/and tamoxifen (5 μmol/L) revealed that the combination treatment significantly increased the early apoptotic cells (Annexin V+/PI−, Fig. 2). The gene discussed is ANXA5; the disease is glioma.