To further characterize the phenotype of nestin+ NPC, day 7 WT and TLR3−/− neurosphere cells were analyzed by FACS for expression of PAX6 and TBR2, two transcription factors expressed in vivo by NPC during mid- and late cortical neurogenesis [50], [51], [52]. Here, TLR3 is linked to nasopharyngeal carcinoma.