ESR1 and metabolic dysfunction-associated steatotic liver disease: Using an ER-positive MCF7 cell line, the ER-luciferase reporter activity was significantly increased by treatment of E2 (P<0.01); however, treatment with WBM extract did not affect ER-luciferase activity (Figure 6B), indicating that the extract does not contain estrogen-like chemicals and ER is not involved in the WBM-mediated protection of the development of NAFLD.