In the present study, SGC7901-siRPL6-2 and AGS-siRPL6-2 showed more effective inhibitory results, which indicated that the SGC7901-siRPL6-2 and AGS-siRPL6-2 cells could be used as a good cell model to clarify the potential role of RPL6 in gene therapy of gastric cancer for clinic. Here, RPL6 is linked to gastric cancer.