Similarly, L-NAME hypertensive rats that do not have cardiac hypertrophy exhibited enhanced expression of Giα-2 and Giα-3 and no changes in Gsα, whereas hypertrophied rats with volume-overload hypertrophy, which do not have hypertension, exhibited decreased levels of Gsα and no augmentation in Giα-2 or Giα-3 proteins. This evidence concerns the gene GNAS and hypertensive disorder.