VPS53 and neoplasm: When the virus-infected tumor cells were subjected to flow cytometric assay after Annexin V-FITC/PI staining, we found that the percentage of Annexin-V positive cells in the Ad.SPDD-HCCS1- or Ad.SPDD-HCCS1HA-infected cell samples was similar to that in the ZD55-HCCS1-infected cell sample, and all of these cell samples had a higher percentage of Annexin-V positive cells than the mock- and Ad.SPDD- infected cell samples (Figure 5B).