RB1 and liver cancer: This adenovirus was generated with the following: the survivin promoter (SP) driving the E1A gene; the deletion of both of the Rb binding domains of E1A; the whole of E1B, including the genes encoding E1B-19K and E1B-55K; and the liver cancer-specific expression cassette AFP-HCCS1-WPRE-SV40, which was inserted upstream of E1A.