In addition, we carried out immunocytochemistry (Supplementary Materials and Methods S1) of Wnt5a in prostate cancer cell lines (LNCaP, 22Rv1 and DU145) after pretreatment with either scrambled or Wnt5a si-RNA (Figure S4A,B,C,D,E). The gene discussed is WNT5A; the disease is prostate cancer.