After treatment with temsirolimus, a compensatory increase in Akt phosphorylation at both sites was detected in the most sensitive endometrial cancer cell lines tested (Fig. 1C), but the primarily resistant cells (KLE) demonstrated no Akt phosphorylation at either site, and another resistant line, Hec50co, showed reduced phosphorylation (Fig. 1D). This evidence concerns the gene AKT1 and endometrial cancer.