To further confirm the relevance of FBP1 promoter CGI hypermethylation in mediating its silencing in human liver, gastric and colon cancer, FBP1 expression and promoter methylation in primary hepatocellular carcinoma, gastric and colon tumor tissues and adjacent non-tumor tissues were analyzed by Real-Time RT-PCR and MSP, respectively. The gene discussed is FBP1; the disease is malignant colon neoplasm.