Figure 4B showed approximately >90% of MNCs expressed mCherry at 2 days of Lentivirus infection. In addition, knockdown of miR-150 in MNCs by lentiviral vector significantly increased CXCR4 expression (Figure 4C). Interestingly, we found that in vivo transplantation of MNCs lacking miR-150 expression (anti-miR-150MNCs) into the irradiated wild type mice resulted in increased number of MNCs in PB released from BM as compared to that of MNCs transducing scramble (ScMNCs), indicating that miR-150 plays a critical role in MNC mobilization in BM through Cxcr4 regulation (Figure 4D). Here, CXCR4 is linked to lentivirus infection.