While the identification of individual genes as potential biomarkers for craniosynostosis is useful, it is also important to discover potential network biomarkers for the disease in addition to individual transcripts like FGF7, SFRP4, and WNT2. To this end, pathway analysis was performed to elucidate gene sets in which individual gene expression changes may be smaller in magnitude, however, en masse these genes may heavily implicate specific pathways. The gene discussed is SFRP4; the disease is craniosynostosis.