Therefore, to evaluate the effect of LL-37 on the IL-8 inducing ability of such LPS preparations, THP-1 monocytes were pre-treated with LL-37 (5 μg/ml) for 1 h prior to stimulation with LPS extracted from P. aeruginosa strains isolated from the lungs of two CF patients with severe lung disease (SE4 and SE22) for 24 h. This evidence concerns the gene CXCL8 and lung disorder.