In established arthritis model, normal percentages of NF-κBp65 were found in mice under Polymerized Collagen, methotrexate, or methotrexate/Polymerized Collagen treatment in the first and second sacrifice (Figures 5(c) and 5(d)), as well as, IκBα levels during second sacrifice (Figure 5(d)). This evidence concerns the gene NFKBIA and Arthritis.