We observed that glioma cells, when treated with pMU and pMC constructs under normoxia, hypoxia, normoxia+IR and hypoxia+IR reduced the expression and activity of hypoxia inducing factor 1 alpha (HIF-1α), when compared with pSV and respective controls in both 4910 and 5310 glioma cells (Figure 5D and Supplementary Figure S3C). This evidence concerns the gene HIF1A and glioma.