CILK1 and infection: Mouse NIH3T3, rat XC, and human TE671 cells expressing the Eco-MLV receptor (TE671/mCAT1) [14] were pretreated with the inhibitors for 5 h, and were inoculated with the Eco- or VSV-pseudotyped MLV vector diluted with fresh medium in absence of the inhibitor to minimize the effects on other steps of the MLV vector infection than entry (such as uncoating and reverse transcription).