To examine the molecular mechanisms underlying 4′-O-methylfisetin-induced melanogenesis, we monitored the mRNA expression of the melanogenic genes, M-type Mitf, A-type Mitf, and Tyrosinase. As shown in Figure 5A, 4′-O-methylfisetin induced these mRNAs in B16F10 melanoma cells. This evidence concerns the gene TYR and melanoma.