SPI1 and B-cell chronic lymphocytic leukemia: To test whether T cells specific for B-CLL-associated antigens had been reactivated, we incubated them with irradiated EBV-negative B-blasts [22] from an HLA-A2-matched donor as a negative control or B-blasts loaded with HLA-A2 restricted peptides derived from B-CLL-associated antigens, namely MDM [23], ETV5 [24] and PU.1 [25].