Studies using animal models such as rodents to understand the developmental origins of metabolic diseases have shown that epigenetic changes in genes correlates with expression changes including metabolic enzymes such as PEPCK, transcriptional factors such as PPARα which regulate fat metabolism and factors associated with insulin action (e.g. PI3 kinase, PKC-ζ), the key regulatory genes which are responsible for bringing these changes are not known [15,33]. This evidence concerns the gene PPARA and Other metabolic disease.