ACTA1 and pulmonary fibrosis: We evaluated lung fibrosis and fibrogenesis employing several complementary methods: i) trichrome stain; ii) Sirius red stain; iii) quantitative Sirius red collagen-binding assay [8],[26]; iv) quantitative hydroxyproline assay; v) quantitative collagen type 1 assay; vi) expression of α-smooth muscle actin (α-SMA) (present in activated LMF); and vii) expression of transforming growth factor (TGF)- β1 (a pro-fibrotic cytokine) [1],[5]–[7],[23],[27].