To further assess if CDC6 is a direct target of miR-886-3p regulation, transfection of the 3′UTR CDC6 wild type vector in thyroid cancer cells with miR-886-3p overexpression showed significant downregulation of luciferase activity suggesting that CDC6 was a direct target of miR-886-3p (Figure 6). This evidence concerns the gene CDC6 and thyroid gland carcinoma.