In fact, while the macroautophagy machinery regulating the covalent association of LC3 to the autophagosomal membranes is dispensable for infection as demonstrated by normal replication of MHV in atg7−/− mouse embryonic fibroblasts (MEFs), the reduction of the intracellular levels of LC3 by siRNA efficiently interferes with MHV replication [45]. The gene discussed is MAP1LC3A; the disease is infection.