CTNNB1 and hepatoblastoma: In contrast, IHC analysis of 20 hepatoblastoma with CTNNB1 mutations or possible deletions showed 5 (25%) were completely negative for Y654-β-catenin (Figure 2a), 14 (70%) had cytoplasmic staining alone (Figure 2b), and only one of 20 (5%) had nuclear expression in addition to cytoplasmic staining (Figure 2c).