In addition, we found that tyrosine phosphorylated proteins coimmunoprecipitated with Nck2 (Nck2 IP) or total Nck (pan-Nck IP) were more abundant in human metastatic melanoma WM1617 cells compared with the counterpart WM278 primary melanoma cells (Figure 8B). This evidence concerns the gene NCK2 and metastatic melanoma.