To rule out that this effect was due to increased cell death in Nck2 depleted melanoma cells, 2 days post transfection we evaluated the percentage of cells with nuclei stained by Trypan Blue and observed no difference between metastatic melanoma cells transfected with control (8.7% ± 2.5) and Nck2 (7.7% ± 1.7) siRNA. The gene discussed is NCK2; the disease is melanoma.