From western blots performed using a rabbit polyclonal antibody that equally recognizes both Nck isotypes (Pan-Nck, Additional file 1) [21], we observed higher levels of Nck proteins in highly metastatic melanoma (WM164, 451Lu, 1205Lu and WM1232) compared with weakly metastatic primary melanoma (WM115 and WM278) and normal melanocyte (FW2294) cell lines (Figure 1A). This evidence concerns the gene NCK1 and melanoma.