To test this, WM278 human primary melanoma cells overexpressing GFP-Nck2 at low (N7) or high (N14) levels, along with parental WM278 cells, WM278 cells overexpressing GFP (C2) and WM1617 human metastatic melanoma cells were injected subcutaneously into CD-1 Nude mice. Here, NCK2 is linked to metastatic melanoma.