On the other hand, apoptotic cells in percent of total number (PI+) (Fig. 4C) in RGNNV-infected cell at 48 h pi by approx 35.5% (Fig. 4C:b) as compare with normal control 1% (Fig. 4B:a), DPI treatment plus RGNNV-infected group 3.5% (Fig. 4C:c) and NAC plus RGNNV-infected group 1.6% (Fig. 4C:d), which reserved a consistent results that antioxidants can rescue cell death from RGNNV infection at middle replication stage (at 48 h pi), but did not at late replication stage (72 h pi), we supposed antioxidants NAC and DPI that may face the half-life problem. The gene discussed is DSP; the disease is infection.