Previous studies demonstrated Akt could regulate the activation of GSK-3β, p70S6K1 and mTOR, and all of them were able to activate Rac1 [45], [46], [47], [48], suggesting that hypoxia stimulates Rac1 activation in breast cancer cells by activating PI3K/Akt signaling. The gene discussed is GSK3B; the disease is breast carcinoma.