Further evidence that these changes in ZIP4 and ZNT4 expression reflect zinc deficiency is provided in panel B. In these experiments, the rat alveolar macrophage cell line, NR8383 was treated in vitro with or without the zinc chelator, N,N,N',N'-tetrakis-(2-pyridyl-methyl) ethylenediamine (TPEN, 5μM) for 24 h, at which time ZIP4 and ZNT4 expression were determined. Here, SLC30A4 is linked to Zinc deficiency.