TNF and polycystic ovary syndrome: In the present study, the frequency of T/T, T/C, and C/C genotypes of -1031(T/C) polymorphism in TNF-α gene was confirmed by sequence analysis and it showed different proportion (p-value = 0.0003, odd ratio (OR) = 2.53) between PCOS and control groups (Figure 2, Table 3).