Pathway express analysis of all the dysregulated genes demonstrated that 19 pathways (Figure 3), including phosphatidylinositol signaling, adherens junction, MAPK signaling, cell cycle, thyroid cancer as well as other cancer pathways, insulin signaling, p53 signaling and apoptosis, were highly perturbed upon silencing of Pax8. The gene discussed is INS; the disease is thyroid cancer.