Taken together, these studies suggest that in vitro treatment with NVP-BEZ235 results in an equivalent decrease in cellular proliferation in two CRC cell lines harboring distinct PIK3CA mutations (HCT116 and DLD-1) as well as in a PIK3CA wild-type colorectal cancer cell (SW480), with no effect on apoptosis. This evidence concerns the gene PIK3CA and colorectal carcinoma.