Real-time PCR for EBV genome, latent (EBER1, EBNA1, LMP1) and lytic (BZLF1) transcripts, and immunohistochemistry for LMP1 and BZLF1 proteins confirmed an active intrathymic EBV infection, further supporting the hypothesis that EBV might contribute to onset or perpetuation of the autoimmune response in MG.Altogether, our results support a role of inflammation and EBV infection as pathogenic features of MG thymus. This evidence concerns the gene PDLIM7 and Epstein-Barr virus infection.